Characterization of the poll allele in Brahman cattle using long-read Oxford Nanopore sequencing

Challenges in Transcriptome Applications Using Oxford Nanopore Sequencing Technology

1School of Biological Sciences, Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln NE 68588, USA 2Department of Computer Science and Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, USA *Corresponding author: Kan Liu, School of Biological Sciences, Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln NE 68588, USA, Tel: +1 402-472...

Long read nanopore sequencing for detection of and HLA

Haplotypes are often critical for the interpretation of genetic laboratory observations into medically actionable findings. Current massively parallel DNA sequencing technologies produce short sequence reads that are often unable to resolve haplotype information. Phasing short read data typically requires supplemental statistical phasing based on known haplotype structure in the population or p...

Oxford Nanopore MinION Sequencing and Genome Assembly

The revolution of genome sequencing is continuing after the successful second-generation sequencing (SGS) technology. The third-generation sequencing (TGS) technology, led by Pacific Biosciences (PacBio), is progressing rapidly, moving from a technology once only capable of providing data for small genome analysis, or for performing targeted screening, to one that promises high quality de novo ...

Robust long-read native DNA sequencing using the ONT CsgG Nanopore system

Background: The ability to obtain long read lengths during DNA sequencing has several potentially important practical applications. Especially long read lengths have been reported using the Nanopore sequencing method, currently commercially available from Oxford Nanopore Technologies (ONT). However, early reports have demonstrated only limited levels of combined throughput and sequence accuracy...

Evaluation of Oxford Nanopore MinION Sequencing for 16S rRNA Microbiome Characterization